Review



monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column  (Merck KGaA)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Merck KGaA monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column
    A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid <t>chromatography</t> <t>(HPLC).</t> B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
    Monolithic Reversed Phase C18 High Performance Liquid Chromatography (Hplc) Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monolithic+reversed-phase+c18+high-performance+liquid+chromatography+column/monolithic+reversed+phase+c18+high+performance+liquid+chromatography++hplc++column/pmc11364864-234-14-24
    Average 90 stars, based on 1 article reviews
    monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Mapping the immunopeptidome of seven SARS-CoV-2 antigens across common HLA haplotypes"

    Article Title: Mapping the immunopeptidome of seven SARS-CoV-2 antigens across common HLA haplotypes

    Journal: Nature Communications

    doi: 10.1038/s41467-024-51959-6

    A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid chromatography (HPLC). B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
    Figure Legend Snippet: A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid chromatography (HPLC). B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

    Techniques Used: Stable Transfection, Purification, High Performance Liquid Chromatography, Derivative Assay, Lysis, Immunoaffinity Purification, Molecular Weight, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Immunopeptidomics

    Related Articles

    Immunopeptidomics:

    Article Title: Methods and compositions for preventing influenza infection
    Article Snippet: Peptides were eluted from the MHC with 10% acetic acid, fractionated on a 4.6 mm internal diameter (i.d.)×100 mm monolithic reversed-phase C18 high-performance liquid chromatography (HPLC) column (Chromolith SpeedROD; Merck Millipore) using an ÄKTAmicro HPLC (GE Healthcare) system, vacuum concentrated, and reconstituted in 0.1% formic acid as described previously (Pymm et al. 2017).

    High Performance Liquid Chromatography:

    Article Title: Methods and compositions for preventing influenza infection
    Article Snippet: Peptides were eluted from the MHC with 10% acetic acid, fractionated on a 4.6 mm internal diameter (i.d.)×100 mm monolithic reversed-phase C18 high-performance liquid chromatography (HPLC) column (Chromolith SpeedROD; Merck Millipore) using an ÄKTAmicro HPLC (GE Healthcare) system, vacuum concentrated, and reconstituted in 0.1% formic acid as described previously (Pymm et al. 2017).



    Similar Products

    monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column  (Merck KGaA)
    90
    Merck KGaA monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column
    A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid <t>chromatography</t> <t>(HPLC).</t> B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
    Monolithic Reversed Phase C18 High Performance Liquid Chromatography (Hplc) Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monolithic+reversed-phase+c18+high-performance+liquid+chromatography+column/monolithic+reversed+phase+c18+high+performance+liquid+chromatography++hplc++column/pmc11364864-234-14-24
    Average 90 stars, based on 1 article reviews
    monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    monolithic reversed-phase c18 high-performance liquid chromatography column  (Merck KGaA)
    90
    Merck KGaA monolithic reversed-phase c18 high-performance liquid chromatography column
    A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid <t>chromatography</t> <t>(HPLC).</t> B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
    Monolithic Reversed Phase C18 High Performance Liquid Chromatography Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monolithic+reversed-phase+c18+high-performance+liquid+chromatography+column/monolithic+reversed+phase+c18+high+performance+liquid+chromatography++hplc++column/us11517617-399-19-29
    Average 90 stars, based on 1 article reviews
    monolithic reversed-phase c18 high-performance liquid chromatography column - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    monolithic reversed-phase (rp) c18 high-performance liquid chromatography (hplc) column  (Merck KGaA)
    90
    Merck KGaA monolithic reversed-phase (rp) c18 high-performance liquid chromatography (hplc) column
    A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid <t>chromatography</t> <t>(HPLC).</t> B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
    Monolithic Reversed Phase (Rp) C18 High Performance Liquid Chromatography (Hplc) Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monolithic+reversed-phase+c18+high-performance+liquid+chromatography+column/lichroprep+rp+18+gel/pmc07726561-144-36-48
    Average 90 stars, based on 1 article reviews
    monolithic reversed-phase (rp) c18 high-performance liquid chromatography (hplc) column - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    chromolith speed rod monolithic reversed-phase (rp) c18 high-performance liquid chromatography (hplc) column  (Merck KGaA)
    90
    Merck KGaA chromolith speed rod monolithic reversed-phase (rp) c18 high-performance liquid chromatography (hplc) column
    A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid <t>chromatography</t> <t>(HPLC).</t> B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
    Chromolith Speed Rod Monolithic Reversed Phase (Rp) C18 High Performance Liquid Chromatography (Hplc) Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monolithic+reversed-phase+c18+high-performance+liquid+chromatography+column/li+chrospher+100++rp+c18++125+x+4+mm++5+%C2%B5m++column/pm30410026-242-18-36
    Average 90 stars, based on 1 article reviews
    chromolith speed rod monolithic reversed-phase (rp) c18 high-performance liquid chromatography (hplc) column - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    4.6-mm (internal diameter) × 50-mm (length) monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column  (Merck KGaA)
    90
    Merck KGaA 4.6-mm (internal diameter) × 50-mm (length) monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column
    A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid <t>chromatography</t> <t>(HPLC).</t> B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
    4.6 Mm (Internal Diameter) × 50 Mm (Length) Monolithic Reversed Phase C18 High Performance Liquid Chromatography (Hplc) Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monolithic+reversed-phase+c18+high-performance+liquid+chromatography+column/4+6+mm++internal+diameter++%C3%97+50+mm++length++monolithic+reversed+phase+c18+high+performance+liquid+chromatography++hplc++column/pmc06499678-349-26-37
    Average 90 stars, based on 1 article reviews
    4.6-mm (internal diameter) × 50-mm (length) monolithic reversed-phase c18 high-performance liquid chromatography (hplc) column - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid chromatography (HPLC). B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

    Journal: Nature Communications

    Article Title: Mapping the immunopeptidome of seven SARS-CoV-2 antigens across common HLA haplotypes

    doi: 10.1038/s41467-024-51959-6

    Figure Lengend Snippet: A Upper panel depicts the large-scale workflow used for the stable transfection and processing of B lymphoblastoid cell lines (BLCL) cells using EF1α-GoI-pIRES-DsRed plasmids containing SARS-CoV-2 genes. Cell pellets (4 x 10 8 to 1 x 10 9 ) were homogenised using a cryomill, lysed, and peptide-HLA complexes immunoaffinity purified using first the W6/32 antibody (HLA class I) and subsequently a mix of SPV-L3, LB3.1, B7/21 antibodies (HLA class II). Peptides were separated from HLA using reverse-phase high-performance liquid chromatography (HPLC). B The lower panel shows the workflow schematic for small-scale protein antigen direct delivery experiments. SARS-CoV-2-derived proteins were electroporated into BLCL and 0.9 ×108 to 1.6 × 108 cells collected 48 h later. Cell pellets were subjected to direct lysis and immunoaffinity purification. Peptides were separated from HLA using 5 kilodalton (kDa) molecular weight cut-off filters. All samples were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and PASEF® on TimsTOF Pro. MHC: Major histocompatibility complex. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

    Article Snippet: For large-scale elution, the peptide-HLA mixtures were fractionated off-line using a 4.6-mm × 100-mm monolithic reversed-phase C18 high-performance liquid chromatography (HPLC) column (Chromolith SpeedROD; Merck Millipore) and an ÄKTAmicro HPLC system (GE Healthcare).

    Techniques: Stable Transfection, Purification, High Performance Liquid Chromatography, Derivative Assay, Lysis, Immunoaffinity Purification, Molecular Weight, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Immunopeptidomics